Paper 13004-58
Paper 13004-58
Determining molar extinction coefficient by high-accuracy optical saturation measurements
On demand | Presented live 9 April 2024 
Abstract
Linear spectrophotometric transmittance measurements are widely used for determining the value of molar extinction coefficient and other basic photophysical parameters of dissolved molecular species. However, such measurements usually require prior information about the molar concentration of the studied chromophores, which in many cases such as e.g. genetically encoded self-maturing species, is not readily available. Here we use wavelength-tunable femtosecond pulses to demonstrate that by performing high-accuracy measurement of small intensity-dependent changes induced in the sample transmittance due to absorption saturation we are able to estimate the extinction coefficient without prior knowledge of the concentration.
Presenter
National Institute of Chemical Physics and Biophysics (Estonia)
Dr. Aleksander Rebane obtained his PhD in 1986 from the Institute of Physics of the Estonian Academy of Sciences (since 1994 the University of Tartu) in then-emerging area of solid-state spectral hole burning spectroscopy and associated holographic coherent data storage- and processing techniques. For this pioneering work he was awarded the International Commission for Optics Prize in 1993. For his early work on developing time-and-space domain holography Dr. Rebane has also been awarded the Leopold Ruzicka Prize of the Swiss Federal Institute of Technology and Y.N. Denisyuk Holography Prize. His current research interest are in the spectroscopy an multi-photon absorption properties of organic and organometallic materials and also in developing advanced experimental techniques for multi-photon spectroscopy using femtosecond laser pulse. Dr. Rebane has published over 200 peer-reviewed papers in international journals devoted to spectroscopy and nonlinear optics.
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