Paper 13115-12
Paper 13115-12
Fluorescence-free Raman spectral measurement using a UV laser
18 August 2024 • 4:40 PM - 5:00 PM PDT
Abstract
We propose a method for separating fluorescence and Raman spectra during photobleaching process. Since the spectral decomposition is based on many spectral data with different fluorescence intensities, the fluorescence and Raman spectra are successfully resolved. As a result, the method effectively reduces the fluorescence interference in Raman spectral measurements. The method was applied to highly fluorescent samples and Raman spectra measured using UV, visible, and near-infrared lasers were compared.
Presenter
Yusuke Shiozaki
Osaka Univ. (Japan), Nanophoton Corp. (Japan)
After completing my master's program at Osaka University in 2011, I joined Nanophoton Corporation. I have been engaged in a wide range of tasks including application, sales, service, manufacturing, software development, and research and development. In particular, as the General Manager of the manufacturing department, I successfully led the outsourcing of production. Since April 2024, I have been conducting research on Raman spectroscopy in the doctoral program at Osaka University, focusing on Raman spectral measurement that avoids fluorescence and imaging data analysis through multivariate analysis.