
Proceedings Paper
Hyperspectral characterization of fluorophore diffusion in human skin using a sCMOS based hyperspectral cameraFormat | Member Price | Non-Member Price |
---|---|---|
$17.00 | $21.00 |
Paper Abstract
Hyperspectral fluorescence imaging is a modality combining high spatial and spectral resolution with increased
sensitivity for low photon counts. The main objective of the current study was to investigate if this technique is a suitable
tool for characterization of diffusion properties in human skin. This was done by imaging fluorescence from Alexa 488
in ex vivo human skin samples using an sCMOS based hyperspectral camera. Pre-treatment with acetone, DMSO and
mechanical micro-needling of the stratum corneum created variation in epidermal permeability between the measured
samples. Selected samples were also stained using fluorescence labelled biopolymers. The effect of fluorescence
enhancers on transdermal diffusion could be documented from the collected data. Acetone was found to have an
enhancing effect on the transport, and the results indicate that the biopolymers might have a similar effect, The
enhancement from these compounds were not as prominent as the effect of mechanical penetration of the sample using a
micro-needling device. Hyperspectral fluorescence imaging has thus been proven to be an interesting tool for
characterization of fluorophore diffusion in ex vivo skin samples. Further work will include repetition of the
measurements in a shorter time scale and mathematical modeling of the diffusion process to determine the diffusivity in
skin for the compounds in question.
Paper Details
Date Published: 10 June 2011
PDF: 8 pages
Proc. SPIE 8087, Clinical and Biomedical Spectroscopy and Imaging II, 808717 (10 June 2011); doi: 10.1117/12.889816
Published in SPIE Proceedings Vol. 8087:
Clinical and Biomedical Spectroscopy and Imaging II
Nirmala Ramanujam; Jürgen Popp, Editor(s)
PDF: 8 pages
Proc. SPIE 8087, Clinical and Biomedical Spectroscopy and Imaging II, 808717 (10 June 2011); doi: 10.1117/12.889816
Show Author Affiliations
J. Hernandez-Palacios, Norsk Elektro Optikk AS (Norway)
Univ. of Oslo (Norway)
I. J. Haug, Norwegian Univ. of Science and Technology (Norway)
Univ. of Oslo (Norway)
I. J. Haug, Norwegian Univ. of Science and Technology (Norway)
Ø. Grimstad, Trondheim Univ. Hospital (Norway)
L. L. Randeberg, Norwegian Univ. of Science and Technology (Norway)
L. L. Randeberg, Norwegian Univ. of Science and Technology (Norway)
Published in SPIE Proceedings Vol. 8087:
Clinical and Biomedical Spectroscopy and Imaging II
Nirmala Ramanujam; Jürgen Popp, Editor(s)
© SPIE. Terms of Use
