
Proceedings Paper
The study of effects of pore architecture in chitosan scaffolds on the fluid flow pattern by Doppler OCTFormat | Member Price | Non-Member Price |
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Paper Abstract
Optimizing and fully understanding the dynamic culture conditions in tissue engineering could accelerate
exploration of this new technique into a promising therapy in the medical field. Scaffolds used in tissue
engineering usually are highly porous with various pore architecture depending on techniques that
manufacture them. Perfusing culture fluid through a scaffold in a bioreactor has proven efficient in
enhancing the exchange of nutrients and gas within cell-scaffold constructs. Upon perfusion, flowing fluid
in pores inevitably produces shear stress on the wall of the pores, which will in turn induce cellular
response for the cells possessing mechanotransducers. Thus, establishing a relationship between perfusion
rate, fluid shear stress and pore architecture in a 3-dimensional cell culture environment is a challenging
task faced by tissue engineers because the same inlet flow rate could induce local variation of flow rate
within the pores. Until recently, there is no proper non-destructive monitoring technique available that is
capable of measuring flow rate in opaque thick objects. In this study, chitosan scaffolds with altered pore
architectures were manufactured by freeze-drying or porogen leaching out or alkaline gelation techniques.
Doppler optical coherence tomography (DOCT) has been used to differentiate the flow rate pattern within
scaffolds which have either elongating pore structure or homogeneous round pore structure. The structural
and flow images have been obtained for the scaffolds. It is found that pore interconnectivity is critically
important in obtaining a steady flow under a given inlet flow rate. In addition, different internal pore
structures affect local flow rate pattern.
Paper Details
Date Published: 24 February 2010
PDF: 6 pages
Proc. SPIE 7566, Optics in Tissue Engineering and Regenerative Medicine IV, 75660J (24 February 2010); doi: 10.1117/12.842266
Published in SPIE Proceedings Vol. 7566:
Optics in Tissue Engineering and Regenerative Medicine IV
Sean J. Kirkpatrick; Ruikang Wang, Editor(s)
PDF: 6 pages
Proc. SPIE 7566, Optics in Tissue Engineering and Regenerative Medicine IV, 75660J (24 February 2010); doi: 10.1117/12.842266
Show Author Affiliations
Ying Yang, Keele Univ. (United Kingdom)
Andreea Iftimia, Keele Univ. (United Kingdom)
Yali Jia, Oregon Health & Science Univ. (United States)
Andreea Iftimia, Keele Univ. (United Kingdom)
Yali Jia, Oregon Health & Science Univ. (United States)
Toby Gould, Keele Univ. (United Kingdom)
Alicia El Haj, Keele Univ. (United Kingdom)
Ruikang K. Wang, Oregon Health & Science Univ. (United States)
Alicia El Haj, Keele Univ. (United Kingdom)
Ruikang K. Wang, Oregon Health & Science Univ. (United States)
Published in SPIE Proceedings Vol. 7566:
Optics in Tissue Engineering and Regenerative Medicine IV
Sean J. Kirkpatrick; Ruikang Wang, Editor(s)
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