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Proceedings Paper

5D-intravital tomography as a novel tool for non-invasive in-vivo analysis of human skin
Author(s): Karsten König; Martin Weinigel; Hans George Breunig; Axel Gregory; Peter Fischer; Marcel Kellner-Höfer; Rainer Bückle; Martin Schwarz; Iris Riemann; Frank Stracke; Volker Huck; Christian Gorzelanny; Stefan W. Schneider
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Paper Abstract

Some years ago, CE-marked clinical multiphoton systems for 3D imaging of human skin with subcellular resolution have been launched. These tomographs provide optical biopsies with submicron resolution based on two-photon excited autofluorescence (NAD(P)H, flavoproteins, keratin, elastin, melanin, porphyrins) and second harmonic generation by collagen. The 3D tomograph was now transferred into a 5D imaging system by the additional detection of the emission spectrum and the fluorescence lifetime based on spatially and spectrally resolved time-resolved single photon counting. The novel 5D intravital tomograph (5D-IVT) was employed for the early detection of atopic dermatitis and the analysis of treatment effects.

Paper Details

Date Published: 22 February 2010
PDF: 6 pages
Proc. SPIE 7555, Advanced Biomedical and Clinical Diagnostic Systems VIII, 75551I (22 February 2010); doi: 10.1117/12.841861
Show Author Affiliations
Karsten König, JenLab GmbH (Germany)
Saarland Univ. (Germany)
Martin Weinigel, JenLab GmbH (Germany)
Hans George Breunig, JenLab GmbH (Germany)
Axel Gregory, JenLab GmbH (Germany)
Peter Fischer, JenLab GmbH (Germany)
Marcel Kellner-Höfer, JenLab GmbH (Germany)
Rainer Bückle, JenLab GmbH (Germany)
Martin Schwarz, Fraunhofer-Institut für Biomedizinische Technik (Germany)
Iris Riemann, Fraunhofer-Institut für Biomedizinische Technik (Germany)
Frank Stracke, Fraunhofer-Institut für Biomedizinische Technik (Germany)
Volker Huck, Univ. of Münster (Germany)
Christian Gorzelanny, Univ. of Münster (Germany)
Stefan W. Schneider, Univ. of Münster (Germany)
Heidelberg Univ. (Germany)

Published in SPIE Proceedings Vol. 7555:
Advanced Biomedical and Clinical Diagnostic Systems VIII
Tuan Vo-Dinh; Warren S. Grundfest M.D.; Anita Mahadevan-Jansen, Editor(s)

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