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Proceedings Paper

Three-dimensional skin imaging using the combination of reflected confocal and multiphoton microscopy
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Paper Abstract

Reflected confocal microscopy has been widely used in clinical application in dermatology. In recent years, multiphoton microscopy has also emerged as an important minimally invasive bioimaging technique for the skin. In this study, we combine reflected confocal microscopy and multiphoton microscopy for skin imaging. In the epidermis, reflected confocal signals are expected to help in delineating cell borders while multiphoton signals provide cytoplasmic morphologies. In the dermis, second harmonic generation signals provide the morphology of collagen fibers. When three-dimensional images are projected, the detailed distribution of cellular component and extracellular matrix in skin can be obtained. Properly developed, this technique is of great potential for in vivo clinical application.

Paper Details

Date Published: 23 March 2007
PDF: 9 pages
Proc. SPIE 6424, Photonic Therapeutics and Diagnostics III, 64240E (23 March 2007); doi: 10.1117/12.699854
Show Author Affiliations
Ming-Gu Lin, National Taiwan Univ. (Taiwan)
Wei-Liang Chen, National Taiwan Univ. (Taiwan)
Wen Lo, National Taiwan Univ. (Taiwan)
Hsin-Yuan Tan, Chang Gung Memorial Hospital (Taiwan)
Tsung-Hua Tsai, Far Eastern Memorial Hospital (Taiwan)
Shiou-Hwa Jee, National Taiwan Univ. Hospital and National Taiwan Univ. (Taiwan)
Sung-Jan Lin, National Taiwan Univ. Hospital and National Taiwan Univ. (Taiwan)
Chen-Yuan Dong, National Taiwan Univ. (Taiwan)

Published in SPIE Proceedings Vol. 6424:
Photonic Therapeutics and Diagnostics III
Henry Hirschberg M.D.; Brian Jet-Fei Wong M.D.; Reza S. Malek M.D.; Kenton W. Gregory M.D.; Nikiforos Kollias M.D.; Bernard Choi; Steen J. Madsen; Guillermo J. Tearney M.D.; Justus F. R. Ilgner M.D.; Haishan Zeng, Editor(s)

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