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Proceedings Paper

Glass based fluorescence reference materials used for optical and biophotonic applications
Author(s): A. Engel; C. Ottermann; U. Resch-Genger; K. Hoffmann; S. Schweizer; J. Selling; J.-M. Spaeth; V. Rupertus
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Paper Abstract

Fluorescence techniques are known for their high sensitivity and are widely used as analytical tools and detection methods for product and process control, material sciences, environmental and biotechnical analysis, molecular genetics, cell biology, medical diagnostics, and drug screening. For routine measurements by fluorescence techniques the existence of an improved quality assurance is one of the basic needs. According to DIN/ISO 17025 certified standards are used for fluorescence diagnostics having the drawback of giving relative values only. Typical requirements onto fluorescence reference materials or standards deal with the verification of the instrument performance as well as the improvement of the data comparability. Especially for biomedical applications fluorescence labels are used for the detection of proteins. In particular these labels consist of nano crystalline materials like CdS and CdSe. The field of Non-Cadmium containing materials is under investigation. In order to evaluate whether glass based materials can be used as standards it is necessary to calculate absolute values like absorption/excitation cross sections or relative quantum yields. This can be done using different quantities of dopands in glass, glass ceramics or crystals. The investigated materials are based on different types of glass, silicate, phosphate and boron glass, which play a dominant role for the absorption and emission mechanism. Additional to the so-called elementary fluorescence properties induced by raw earth elements the formation of defects lead to higher cross sections additionally. The main investigations deal with wavelength accuracy and lifetime of doped glasses, glass ceramics and crystalline samples. Moreover intensity patterns, homogeneity aspects and photo stability will be discussed.

Paper Details

Date Published: 14 April 2006
PDF: 10 pages
Proc. SPIE 6191, Biophotonics and New Therapy Frontiers, 619110 (14 April 2006); doi: 10.1117/12.663627
Show Author Affiliations
A. Engel, Schott AG (Germany)
C. Ottermann, Schott AG (Germany)
U. Resch-Genger, Federal Institute for Materials Research and Testing (Germany)
K. Hoffmann, Federal Institute for Materials Research and Testing (Germany)
S. Schweizer, Univ. of Paderborn (Germany)
J. Selling, Univ. of Paderborn (Germany)
J.-M. Spaeth, Univ. of Paderborn (Germany)
V. Rupertus, Schott AG (Germany)

Published in SPIE Proceedings Vol. 6191:
Biophotonics and New Therapy Frontiers
Romualda Grzymala; Olivier Haeberle, Editor(s)

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