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Proceedings Paper

Using a continuum of light in STED confocal microscopy
Author(s): C. Courvoisier; R. Giust
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Paper Abstract

In this work, we numerically show that a continuum of light, generated by a femtosecond laser and a microstrured fiber (MF) can be used as tunable source for STED microscopy. The envisaged setup is composed by a microscope on which such a continuum is inserted. The spectral excitation and STED bands selection is performed by a monochromator based on an acousto-optic tunable filter (AOTF). Pulse temporal shapes fluctuations and intensity fluctuations effects are investigated. Those fluctuations are consistent with the experimental measurements. Temporal variations have little influence on the STED efficiency, whereas intensity fluctuations have much more effects.

Paper Details

Date Published: 14 April 2006
PDF: 10 pages
Proc. SPIE 6191, Biophotonics and New Therapy Frontiers, 619108 (14 April 2006); doi: 10.1117/12.661721
Show Author Affiliations
C. Courvoisier, Univ. de Franche-Comté, Institut FEMTO-ST, CNRS (France)
R. Giust, Univ. de Franche-Comté, Institut FEMTO-ST, CNRS (France)

Published in SPIE Proceedings Vol. 6191:
Biophotonics and New Therapy Frontiers
Romualda Grzymala; Olivier Haeberle, Editor(s)

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