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Proceedings Paper

Raman study of analysis for the states of maturation of neural cell
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Paper Abstract

The combination of confocal micro-Raman spectroscopy and multivariate analysis is carried out for analysis of maturation of neurons. This study suggests that Raman data reflects the stages of neural maturation which relates with the expression of new neural function such as spontaneous activity. Neurons obtained from a hippocampus of rat embryos are cultured in a dish with quartz bottom. According to the previous electrophysiological study, matured neural cell network showed regulated pulsation with interval of several seconds without any stimulation. It suggested that alterations in the molecular composition took place in the cell. The Raman measurements are carried out to observe this alteration along with the maturing process of neurons. The spectra of live neural cells measured after 2, 8, 15, 30, 45, 60, 75, 90, 105 and 120 days of culturing are analyzed by principal component analysis (PCA). The result shows several groups suggesting the maturation scheme which is observed by the electrophysiological studies. It demonstrates that the maturation process of neural cells can be monitored by Raman spectroscopy.

Paper Details

Date Published: 4 March 2014
PDF: 6 pages
Proc. SPIE 8939, Biomedical Vibrational Spectroscopy VI: Advances in Research and Industry, 893914 (4 March 2014); doi: 10.1117/12.2039073
Show Author Affiliations
Kosuke Hashimoto, Kwansei Gakuin Univ. (Japan)
Suguru N. Kudoh, Kwansei Gakuin Univ. (Japan)
Hidetoshi Sato, Kwansei Gakuin Univ. (Japan)

Published in SPIE Proceedings Vol. 8939:
Biomedical Vibrational Spectroscopy VI: Advances in Research and Industry
Anita Mahadevan-Jansen; Wolfgang Petrich, Editor(s)

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