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Proceedings Paper

Assay for optical determination of biogenic amines using microtiterplates
Author(s): Polona Nedeljko; Matejka Turel; Aleksandra Lobnik
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Paper Abstract

Direct determination of catecholamine noradreanaline (NOR) is presented using o-phthaldialdehyde (OPA) as an indicator reagent. The fluorescent assay in which OPA forms with NOR a fluorescent complex (OPA-NOR) can be monitored at neutral, physiological conditions (pH 7) and performed in microtiterplates. The determination of NOR is optimal in the concentration range from 4.0×10-7 to 1.0×10-5 M and limit of detection is 4.0×10-7 M. The OPA-NOR complex maximum intensity is reached within 5 minutes. Dopamine and adrenaline could not be determined using the same approach.

Paper Details

Date Published: 3 May 2013
PDF: 7 pages
Proc. SPIE 8774, Optical Sensors 2013, 87741A (3 May 2013); doi: 10.1117/12.2019486
Show Author Affiliations
Polona Nedeljko, Institute for Environmental Protection and Sensors, Ltd. (Slovenia)
Matejka Turel, Institute for Environmental Protection and Sensors, Ltd. (Slovenia)
Aleksandra Lobnik, Institute for Environmental Protection and Sensors, Ltd. (Slovenia)
Univ. of Maribor (Slovenia)

Published in SPIE Proceedings Vol. 8774:
Optical Sensors 2013
Francesco Baldini; Jiri Homola; Robert A. Lieberman, Editor(s)

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