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Conference 13006 > Paper 13006-9
Paper 13006-9

subcellular organelle metabolic correlation with super-resolution chemical imaging (Invited Paper)

8 April 2024 • 15:30 - 16:00 CEST | Etoile B, Niveau/Level 1

Abstract

We've established a nonlinear multimodal imaging system that incorporates stimulated Raman Scattering (SRS), multiphoton fluorescence (MPF), and second harmonic generation (SHG) to explore the connections between metabolic activities and the distribution of metabolites in cells and tissues. Furthermore, we've devised the Adam-based Pointillism Deconvolution (A-PoD) and Correlation Coefficient Mapping (CoCoMap) algorithms, enabling a deeper insight into the simultaneous recording and regulation of various metabolic processes within super-resolved images of nanoscale Regions of Interest (ROIs). In our pursuit of specifically identifying signals originating from distinct subcellular organelles, we've introduced a pioneering clustering algorithm known as Multi-SRS reference matching (Multi-SRM). This approach has the potential to improve early disease detection, prognosis, the evaluation of therapeutic effects, and our comprehension of the mechanisms underpinning aging and biomedicine.

Presenter

Hongje Jang
Univ. of California, San Diego (United States)
Dr. Hongje Jang earned his Ph.D. in Physics Department at University of Goettingen. Currently, he is a postdoc research scientist at the Shi Lab in the Shu Chien-Gene Lay Department of Bioengineering at UC San Diego. He developed super-resolution stimulated Raman scattering (SRS) microscopy using Adam-based Pointillism Deconvolution (A-POD) (Nature Methods 2023), which became a significant contribution to the high resolution imaging field. This approach enabled the quantitative examination of nanoscopic biomolecular distribution and subcellular metabolic activity in cells and tissues. Dr. Jang will continue developing multimodal nanoscopic imaging technolgies and analysis methods for studying metabolic dynamics in aging and diseases.
Presenter/Author
Hongje Jang
Univ. of California, San Diego (United States)
Author
Univ. of California, San Diego (United States)
Author
Yajuan Li
Univ. of California, San Diego (United States)
Author
Yu Ping
Univ. of California, San Diego (United States)
Author
Univ. of California, San Diego (United States)