See you in two years!
11-16 April 2026
Conference 13006 > Paper 13006-58
Paper 13006-58

Towards label-free liquid biopsy: combining machine learning and tomographic phase imaging flow cytometry for the identification of tumor cells

11 April 2024 • 13:20 - 13:40 CEST | Etoile B, Niveau/Level 1

Abstract

Liquid biopsy is an emerging and promising biomedical tool that aims to the early cancer diagnosis and the definition of personalized therapies in non-invasive and cost-effective way. Recently, tomographic phase imaging flow cytometry (TPIFC) has been developed as a technique for the reconstruction of the 3D volumetric distribution of the refractive indices (RIs) of single cells flowing along a microfluidic channel. Here we investigate the possibility of exploiting the 3D dataset of single cells recorded by TPIFC to feed a machine learning model, in order to recognize tumor cells with respect to a background of monocytes, which are the most similar cells among the WBCs in terms of morphology. Reported results aim to emulate a real scenario for the label-free liquid biopsy based on the machine learning-powered identification of circulating tumor cells within a blood sample imaged by the TPIFC system.

Presenter

Istituto di Scienze Applicate e Sistemi Intelligenti "Eduardo Caianiello", Consiglio Nazionale delle Ricerche (Italy)
Presenter/Author
Istituto di Scienze Applicate e Sistemi Intelligenti "Eduardo Caianiello", Consiglio Nazionale delle Ricerche (Italy)
Author
CEINGE-Biotecnologie Avanzate (Italy)
Author
Univ. degli Studi di Bologna (Italy)
Author
Giusy Giugliano
Istituto di Scienze Applicate e Sistemi Intelligenti "Eduardo Caianiello", Consiglio Nazionale delle Ricerche (Italy)
Author
Istituto di Scienze Applicate e Sistemi Intelligenti "Eduardo Caianiello", Consiglio Nazionale delle Ricerche (Italy)
Author
Univ. degli Studi di Napoli Federico II (Italy)
Author
CEINGE-Biotecnologie Avanzate (Italy)
Author
CEINGE-Biotecnologie Avanzate (Italy)
Author
Univ. degli Studi di Bologna (Italy)
Author
Univ. degli Studi di Bologna (Italy)
Author
Univ. degli Studi di Bologna (Italy)
Author
Istituto di Scienze Applicate e Sistemi Intelligenti "Eduardo Caianiello", Consiglio Nazionale delle Ricerche (Italy)
Author
Istituto di Scienze Applicate e Sistemi Intelligenti "Eduardo Caianiello", Consiglio Nazionale delle Ricerche (Italy)
Author
Univ. degli Studi di Napoli Federico II (Italy)
Author
Univ. degli Studi di Napoli Federico II (Italy)
Author
Univ. degli Studi di Napoli Federico II (Italy)
Author
Pasquale Memmolo
Istituto di Scienze Applicate e Sistemi Intelligenti "Eduardo Caianiello", Consiglio Nazionale delle Ricerche (Italy)
Author
Istituto di Scienze Applicate e Sistemi Intelligenti "Eduardo Caianiello", Consiglio Nazionale delle Ricerche (Italy)