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11-16 April 2026
Conference 13006 > Paper 13006-22
Paper 13006-22

Creation of evanescent patterns at micron resolution by scanning an evanescent spot

9 April 2024 • 11:20 - 11:40 CEST | Etoile B, Niveau/Level 1

Abstract

Total internal reflection fluorescence (TIRF) microscopy is a well-known technique allowing to confine the light close to the surface of a glass substrate. This axial confinement is based on the generation of evanescent waves. In TIRF microscopy however, there is no control of the light intensity in the transverse plane. Here, we propose a method to create evanescent patterns, which uses a fast-switching digital micro-mirror device to generate and scan an evanescent spot at multiple positions on the sample plane. In this way, patterns confined in the three dimensions of space can be produced in a fraction of second. This method would allow better spatial control in photo-activation or photo-conversion experiments in living cells, e.g. to target processes located at cell membranes.

Presenter

Marc Grosjean
Lab. Interdisciplinaire de Physique (France)
I am a third year student from the University of Grenoble Alpes (UGA) at the Laboratoire interdisciplinaire de physique (LIPhy). I have a bachelor's and master's degree in physics from UGA, and I am currently working on a thesis on evanescent light patterning for biological applications.
Presenter/Author
Marc Grosjean
Lab. Interdisciplinaire de Physique (France)
Author
Irene Wang
Lab. Interdisciplinaire de Physique (France)
Author
Olivier Destaing
Institut pour l'Avancée des Biosciences (France)
Author
Institut pour l'Avancée des Biosciences (France)
Author
Institut pour l'Avancée des Biosciences (France)