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11-16 April 2026
Conference 13006 > Paper 13006-109
Paper 13006-109

Extending two photon scanning microscopy for imaging on novel species. Brain-wide in-vivo imaging in Xenopus tadpoles

10 April 2024 • 14:20 - 14:40 CEST | Londres 1/Salon 8, Niveau/Level 0

Abstract

In neuroscience, two photon scanning microscopy is commonly used to record brain activity in species that differ greatly in brain size and their properties and distributions of neurons. Accordingly, tailoring the properties of the imaging system to the experimental model in question is critical. These include adjustments in the size of the field-of-view, the shape and curvature of the scan-plane, or adjustment in excitation PSF sizes. Here, we report our progress to optimising these and other imaging parameters for high-signal-to-noise imaging of whole-brain neuronal activity in the large nervous system of Xenopus laevis tadpoles with comparable quality compared to what is currently possible in juvenile zebrafish.

Presenter

Filip janiak
University of Sussex (United Kingdom)
Filip Janiak is a Research Fellow at University of Sussex, UK. His work is focusing on development of the optical methods to record in-vivo brain activity of different species. He got his MSc and PhD in a field of solid-state physics at Wroclaw University of Science and Technology, Poland. Before he moved to work in the UK, he spent 3 years working in neuroscientific labs in Belgium (NERF, Imec, Leuven) and Norway (Kavli Institute for System Neuroscience, NTNU, Trondheim).
Presenter/Author
Filip janiak
University of Sussex (United Kingdom)
Author
Carola Yovanovich
University of Sussex (United Kingdom)
Author
Michael Forsthofer
University of Sussex (United Kingdom)
Author
David Vijatovic
IST Austria (Austria)
Author
Lora Sweeney
IST Austria (Austria)
Author
Tom Baden
University of Sussex (United Kingdom)