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11-16 April 2026
Conference 13006 > Paper 13006-108
Paper 13006-108

Sensing forces in cells using fluorescence FRET microscopy and optical tweezers

10 April 2024 • 14:00 - 14:20 CEST | Londres 1/Salon 8, Niveau/Level 0

Abstract

Forces inside cells play a fundamental role in cell behavior, for example in cancer cell migration. We focus on the vinculin protein which is involved in the stabilization of cell adhesion. Through fluorescence transfer (FRET), forces within vinculin can be measured with picoNewton sensitivity. We measure these internal forces while applying a calibrated external force with a laser-based optical tweezer via a microbead attached to the cell. Our most recent results using fibroblast cells show that the force applied with the optical tweezer induces the recruitment of vinculin and the formation of focal adhesions on the bead within a few minutes. Once the bead is attached to the cell, we record its trajectory and infer the force exerted by the cell. We correlate this force with the FRET efficiency of the force sensor.

Presenter

Camille Dubois
Institut d'Optique Graduate School, Université Paris-Saclay, CNRS, Laboratoire Charles Fabry, Palaiseau, France (France)
I am a 3rd year PhD student in Biophotonics at Laboratoire Charles Fabry, Université Paris-Saclay. I am performing my dissertation work with Dr. Westbrook. My research interest focuses on the cross-section of optics and biophysics. I have received a master’s degree in physics from Institut d’Optique and a master’s degree in light-matter interaction from Université de Bordeaux.
Presenter/Author
Camille Dubois
Institut d'Optique Graduate School, Université Paris-Saclay, CNRS, Laboratoire Charles Fabry, Palaiseau, France (France)
Author
Institut d'Optique Graduate School, Université Paris-Saclay, CNRS, Laboratoire Charles Fabry (France)
Author
Rutgers University, Department of Biomedical Engineering, Piscataway, New Jersey, United States (United States)
Author
Institut de Chimie Physique, Université Paris-Saclay, CNRS, Orsay, France (France)
Author
Institut des Sciences Moléculaires d'Orsay, Université Paris-Saclay, CNRS, Orsay, France (France)