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Conference 13006 > Paper 13006-46
Paper 13006-46

Optical coherence phase microscopy with refractive index measurement of Leydig cell using negative axicon probe

10 April 2024 • 14:50 - 15:10 CEST | Etoile B, Niveau/Level 1


In this work, the negative axicon probe is employed in an optical coherence phase microscopy (OCPM) system. The phase retrieval is done using conventional Hilbert transform for imaging and the Fresnel relation is used for refractive index measurement. In which, the reflected powers from air–glass and sample–glass interfaces are estimated using a fast-Fourier transform. Our OCPM system provides a phase sensitivity of ∼0.28 mrad and optical path length sensitivity of ∼23pm in air. This system is explored for phase imaging and refractive index measurement of the human male Leydig cells. A typical dimension of a Leydig cell from our system is ~19μm whereas the mean refractive index across a cell is found to be 1.3535. This system has potential to produce the phase imaging along with refractive index measurement of human male Leydig cell.


CSIR - Central Scientific Instruments Organisation (India)
Presently, I am working as a Research Associate at Micro & Nano Optics Centre, Central Scientific and Instruments Organisation (CSIO) Campus, Chandigarh, India. I have done my PhD thesis on " Bio-sample imaging with refractive index measurement using common- path low coherence interferometry and Bessel beam" from Academy of Scientific & Innovative Research (AcSIR), India under the supervision of Dr Samir K Mondal. Till now, I have published 9 research articles in reputed SCI journals. I have presented/published 12 papers in renowned conferences. I am an active member of SPIE, Optica and IEEE. I was a recipient of SPIE Officer Travel Grant for SPIE Photonics West 2019 and IEEE Photonics Student & Young Professionals grantee for IEEE Photonics Conference 2022. My current research interest includes Optical Coherence Tomography, Phase Microscopy, Signal & image Processing, Light-Tissue Interaction, Whispering Gallery modes, Optical Sensors & Instrumentation, Axicon, and Bessel beam.
CSIR - Central Scientific Instruments Organisation (India)
Manish Rohilla
Postgraduate Institute of Medical Education & Research, Chandigarh (India)
Samir K. Mondal
CSIR - Central Scientific Instruments Organisation (India)