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11-16 April 2026
Conference 13006 > Paper 13006-24
Paper 13006-24

Near infrared laser provoques focus variation in a confocal microscope

9 April 2024 • 12:00 - 12:20 CEST | Etoile B, Niveau/Level 1

Abstract

Precision and stability in confocal microscopy are vital for maintaining image clarity and preventing misinterpretation of radiometric fluctuations as changes in fluorescence intensity within the sample. This study discusses a focus variation induced by an 810-nm continuous wave laser integrated into the optical path of an inverted confocal microscope with a 60x oil immersion objective. Activation or deactivation of the laser leads to a drift in the focus position towards lower or higher values of the vertical coordinate z, respectively. The maximum observed drift is 2.25 μm, occurring with a 40 mW laser power at the sample over a 600-second exposure time. The temporal behavior of the focus position follows exponential curves resembling temperature fluctuations associated with a heat source. Our analysis strongly indicates that the focus drift is attributable to the heating of the immersion oil.

Presenter

Univ. Nacional Autónoma de México (Mexico)
Remy Avila obtained his PhD in Physics in 1998 at the University of Nice, France. Since 1999 he joined the National Autonomous University of Mexico (UNAM) as a researcher. Dr. Ávila holds a senior research position at the Center for Applied Physics and Advanced Technology (CFATA), and is head of the Biophotonics lab. He is also head of graduate studies at CFATA. Dr. Avila has published over 70 indexed and supervised more than 20 thesis. The current research fields followed by Dr. Avila are the study of mechanical properties of cancer cells, the development of novel optical tweezers systems and the development of 3D collagen hydrogels as mimics of the extracellular matrix.
Presenter/Author
Univ. Nacional Autónoma de México (Mexico)
Author
Melissa Matrecitos-Avila
Univ. Nacional Autónoma de México (Mexico)
Author
Reinher Pimentel-Domínguez
Univ. Nacional Autónoma de México (Mexico)
Author
Elisa Tamariz
Univ. Veracruzana (Mexico)
Author
ICFO - Institut de Ciències Fotòniques (Spain)
Author
Univ. Nacional Autónoma de México (Mexico)