7 - 11 April 2024
Strasbourg, France
Conference 13006 > Paper 13006-101
Paper 13006-101

Monitoring antibiotic susceptibility of Escherichia coli through analysis of fluorescence

On demand | Presented live 9 April 2024


This study investigates an indirect measurement of the antibiotic susceptibility of Escherichia coli (E. coli) strain ATCC 25922 by analyzing sample background fluorescence at 455 nm excitation and 530 nm emission wavelengths. The study takes a practical approach by exposing the bacterial culture, suspended in Luria-Bertani (LB) broth medium, to various concentrations of ampicillin and analyzing the corresponding fluorescence intensities of the inhibitory and non-inhibitory ranges of antibiotic concentrations. The variations in fluorescence intensities indicate probable antibiotic sensitivity. The results are validated with gold standards such as the broth microdilution method.


Indian Institute of Technology Madras (India)
Mr. Kiran Philip Isaac is pursuing his PhD in the Department of Applied Mechanics and Biomedical Engineering at the Indian Institute of Technology Madras, India. He completed his master's from IIT Madras and his undergraduate from Sathyabama University, India. His research interests are spectroscopy techniques, laser speckle imaging, and fluorescence, which are used to study antibiotic susceptibility.
Indian Institute of Technology Madras (India)
Indian Institute of Technology Madras (India)