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Conference 13006 > Paper 13006-10
Paper 13006-10

Measuring chemical changes during aging in vivo with stimulated Raman scattering microscopy

8 April 2024 • 16:00 - 16:20 CEST | Etoile B, Niveau/Level 1

Abstract

Aging related biological mechanisms are often difficult to probe in situ without exogenous fluorophores. Here, we leverage label-free stimulated Raman scattering (SRS) microscopy to provide new insights into aging in C. elegans. We demonstrate multispectral SRS imaging of whole worms in vivo with quantitative chemical insights across different ages. We show that both lipid and protein synthesis and compartmentalization are associated with aging in worms. We additionally use SRS in combination with simultaneous two-photon fluorescence imaging to characterize the putatively aberrant protein accumulation. Moreover, we observe notable SRS image differences when worms are subjected to calorie restriction, suggesting a promising avenue towards understanding calorie-restriction’s enhancing effects on longevity when coupled to proteomic and metabolomic analysis.

Presenter

Univ. of California, Berkeley (United States)
Bryce is a postdoctoral scholar at the University of California Berkeley working with professor Aaron Streets in the bioengineering department. He is interested in leveraging nonlinear light-matter interactions to study biological systems at molecular to tissue scales. He completed his PhD in Chemistry in May 2022 with Dan Fu at the University of Washington where he worked on stimulated Raman scattering (SRS) microscopy and developed deep learning algorithms to augment SRS image analysis. He previously graduated from Indiana University with a B.S. in Chemistry with distinction and honors where he worked with Megan Thielges on probing protein binding dynamics using infrared spectroscopy.
Presenter/Author
Univ. of California, Berkeley (United States)
Author
Univ. of California, Berkeley (United States)
Author
Univ. of California, Berkeley (United States)
Author
Aaron Streets
Univ. of California, Berkeley (United States)