See you in two years!
11-16 April 2026
Conference 13006 > Paper 13006-105
Paper 13006-105

IN2SIGHT: a microstructured window for label-free non-linear imaging in vivo.

11 April 2024 • 15:50 - 16:10 CEST | Etoile B, Niveau/Level 1

Abstract

Tissue histopathology, reliant on costly and time-consuming hematoxylin and eosin (H&E) staining of thin tissue slices, faces limitations. Label-free non-linear optical microscopy in vivo presents a solution, allowing work on fresh samples. Implantable microstructures prove effective for systematic longitudinal in vivo studies of immunological responses to biomaterials using label-free non-linear optical microscopy. Employing two-photon laser polymerization, we implanted a matrix of 3D lattices in the chorioallantoic membrane of chicken embryos, establishing a 3D reference frame for cell counting. H&E analysis is compared to label-free in vivo non-linear excitation imaging for cell quantification and identifying granulocytes, collagen, and microvessels. Preliminary results in higher animal models demonstrate the transformative potential of this approach, offering an alternative to conventional histopathology for validating biomaterials in in vivo longitudinal studies.

Presenter

Univ. degli Studi di Milano-Bicocca (Italy)
Giuseppe Chirico, has a Ms. in Physics, PhD in Biophysics and is full professor of Applied Physics (Univ. Milano-Bicocca, Milano, I). His research has been in laser spectroscopy and microscopy for biology and medical biotechnology. Recent research is on photo- and fluorescence correlation spectroscopies and applications to study of the structure and dynamics of proteins and nanoparticles interacting with cells and in-vivo deep tissue imaging by non-linear excitation microscopy. # Publications: 206; # Citations 3955 H index 32
Application tracks: EU-funded Research
Presenter/Author
Univ. degli Studi di Milano-Bicocca (Italy)
Author
Univ. degli Studi di Milano-Bicocca (Italy)
Author
Davide Panzeri
Univ. degli Studi di Milano-Bicocca (Italy)
Author
Politecnico di Milano (Italy)
Author
I.R.C.C.S. Ospedale San Raffaele (Italy)
Author
Politecnico di Milano (Italy)
Author
Istituto di Fotonica e Nanotecnologie, Consiglio Nazionale delle Ricerche (Italy), Politecnico di Milano (Italy)
Author
Istituto di Fotonica e Nanotecnologie, Consiglio Nazionale delle Ricerche (Italy), Politecnico di Milano (Italy)
Author
Maddalena Collini
Univ. degli Studi di Milano-Bicocca (Italy)
Author
Giulio Cerullo
Politecnico di Milano (Italy)
Author
Politecnico di Milano (Italy)