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Conference 13006 > Paper 13006-78
Paper 13006-78

High resolution and fast chemical imaging using widefield optical photothermal infrared microscope

On demand | Presented live 9 April 2024

Abstract

Label-free infrared (IR) imaging reveals chemical bonds in specimens, but its limitations include a spatial resolution of approximately 5 μm. The advent of optical photothermal infrared (OPTIR) microscopy extends molecular fingerprint imaging by one order of magnitude near 500 nm. However, traditional scanning OPTIR faces a challenge of imaging speed. To improve speed and throughput, we employ pulsed visible probe light for widefield detection of transient photothermal responses induced by mid-infrared pulses. Our time-gated camera technique achieves sub-microsecond temporal resolution. We successfully imaged polystyrene beads, submicron SU8 polymer etchings, and mouse brain tissue samples using our widefield OPTIR microscope. The system excels in probe-dependent temporal and submicron spatial resolution, operating at 100 Hz over a 50 μm diameter field of view, while maintaining reasonable spectral fidelity. This enhanced widefield OPTIR microscopy promises rapid, label-free chemical imaging for biological samples and high-throughput screening applications.

Presenter

Abbe Ctr. of Photonics, Friedrich-Schiller-Univ. Jena (Germany), Leibniz-Institut für Photonische Technologien e.V. (Germany)
Subham Adak is a graduate student pursuing M.Sc. Photonics from Abbe School of Photonics (Friedrich-Schiller-University Jena) in Jena, Germany. His scientific interests focus on microscopy and spectroscopy for clinical applications. Currently, he is working on his master thesis project under the supervision of Dr. Christoph Krafft and Prof. Juergen Popp at Leibniz Institute of Photonic Technology in Jena, Germany. Previously, he completed his bachelor's degree in Physics from Birla Institute of Technology Mesra in India, where he worked on terahertz plasmonic biosensing, multimodal non-linear microscopy, and fluorescence lifetime imaging (FLIM) microscopy.
Application tracks: EU-funded Research
Presenter/Author
Abbe Ctr. of Photonics, Friedrich-Schiller-Univ. Jena (Germany), Leibniz-Institut für Photonische Technologien e.V. (Germany)
Author
Leibniz-Institut für Photonische Technologien e.V. (Germany)
Author
Abbe Ctr. of Photonics, Friedrich-Schiller-Univ. Jena (Germany), Leibniz-Institut für Photonische Technologien e.V. (Germany)
Author
Leibniz-Institut für Photonische Technologien e.V. (Germany)