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Conference 13006 > Paper 13006-74
Paper 13006-74

Experimental validation of a theoretical model for photoacoustic pump probe spectroscopy

9 April 2024 • 18:10 - 20:00 CEST | Galerie Schweitezer, Niveau/Level 0

Abstract

Genetically expressed fluorescent proteins are attractive contrast agents for molecular photoacoustic tomography. Pump-probe excitation, relying on the excitation of fluorescent proteins with a pump pulse and the subsequent stimulation of fluorescent emissions using a time-delayed probe pulse, is a simple experimental method that has been shown to provide fluorophore specific contrast. We developed a theoretical model to simulate photoacoustic pump probe signal generation in fluorophores. The results were validated experimentally in solutions of red fluorescent proteins and dyes using a photoacoustic spectrometer. The modeling results show good agreement with experimental data when scanning the pump wavelength across the absorption spectrum of fluorophores and when the pump probe time delay is varied. The results suggest that fluorescent proteins can be used as biosensors in PA imaging to measure biophysical parameters within tissue such as pH.

Presenter

Farzin Ghane Golmohamadi
Martin-Luther-Univ. Halle-Wittenberg (Germany)
Presenter/Author
Farzin Ghane Golmohamadi
Martin-Luther-Univ. Halle-Wittenberg (Germany)
Author
Martin-Luther-Univ. Halle-Wittenberg (Germany)
Author
Martin-Luther-Univ. Halle-Wittenberg (Germany)
Author
Martin-Luther-Univ. Halle-Wittenberg (Germany)
Author
Patrick Galert
Martin-Luther-Univ. Halle-Wittenberg (Germany)
Author
Martin-Luther-Univ. Halle-Wittenberg (Germany)