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Conference 13006 > Paper 13006-91
Paper 13006-91

Enhancing the sensitivity of small particle detection in flow cytometry via optical coherence tomography

9 April 2024 • 18:10 - 20:00 CEST | Galerie Schweitezer, Niveau/Level 0

Abstract

This research focuses on enhancing the sensitivity of flow cytometry for the detection of extracellular vesicles (EVs), particularly those smaller than 100-200 nm. Extracellular vesicles play a crucial role in cellular waste disposal and intercellular communication, serving as disease biomarkers. Conventional flow cytometers often lack the required sensitivity for accurately detecting such small EVs. The study proposes the integration of Optical Coherence Tomography (OCT) with flow cytometry to improve sensitivity and reduce background signals. We first demonstrate the proof of concept with a commercial OCT system and conduct a comparative analysis alongside established commercial flow cytometers using a solution of polystyrene beads with known sizes. We determined the lower limit of detection (LOD) for the current OCT system to be a polystyrene bead of 600 nm diameter. Using this LOD, we describe potential hardware and software optical and fluidic modifications and calculate the potential LOD of an adapted OCT system to be the detection of an EV of 100 nm in diameter.

Presenter

Kirill Buiankin
Amsterdam UMC (Netherlands)
Kirill Buiankin obtained his BS and MS at Physics at Lomonosov Moscow State university. Now he is a PhD candidate at Amsterdam University Medical Center at the department of Biomedical Engineering and Physics. His research topic concerns improving the sensitivity of flow cytometers for small particle detection.
Application tracks: EU-funded Research
Presenter/Author
Kirill Buiankin
Amsterdam UMC (Netherlands)
Author
Edwin van der Pol
Amsterdam UMC (Netherlands)
Author
Amsterdam UMC (Netherlands)