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11-16 April 2026
Conference 13006 > Paper 13006-42
Paper 13006-42

Development of a combined multi-spectral optoacoustic microscopy and optical coherence tomography imaging instrument for mapping multiple chromophores in biological tissues (Invited Paper)

10 April 2024 • 13:10 - 13:40 CEST | Etoile B, Niveau/Level 1

Abstract

This report presents a state-of-the-art multimodality imaging device that combines multi-spectral optoacoustic microscopy (OAM) and optical coherence tomography (OCT) to chart absorbers in live tadpoles (Xenopus laevis) accurately. The OAM channel captures maps of five internal contrast agents: melanin, hemoglobin, collagen, glucose, and lipids. A novel method was developed to achieve this by assuming that each voxel in the 3D-OAM image exhibits a single chromophore contributing to the optoacoustic signal. The device is powered by a single optical source (SuperK Compact, NKT Photonics) that operates across an ultra-wide spectral range of 450 to 2400 nm. The set-up was optimized by minimizing optical aberrations and attenuation on optical components to stimulate the sample effectively. Using optical pulses of 2 ns duration and a repetition rate of 20 kHz, the device imaged tadpoles in their embryonic stage at multiple wavelengths, using narrow spectral windows of 25 nm bandwidth within the broad spectrum of the supercontinuum source at a time. In addition, an ultra-high-resolution OCT imaging channel operating at 1300 nm (spectral bandwidth 180 nm) was created and incorporated into the device. The OCT channel, also powered by a commercial supercontinuum source (SuperK EXTREME EXR9, NKT Photonics), was used for guidance purposes and to help determine the location of the chromophores.

Presenter

Univ. of Kent (United Kingdom)
Dr Adrian Bradu is a Senior Lecturer in Applied Optics at the School of Physics and Astronomy at the University of Kent, Canterbury, UK. Before starting his academic career, Dr Bradu worked as a Researcher and Researcher Co-investigator, focusing on various subjects focused on adaptive optics, and OCT. Dr. Bradu's current research interests focus on OCT technology and the development of a line of research on photoacoustic imaging. He possesses considerable expertise and knowledge in fields such as confocal microscopy, imaging of the eye, fibre optics technology, image and data acquisition and processing, software development, etc. Dr. Bradu has published over 100 articles in top optics journals and over 150 conference proceedings. He has shared the results of his research work in over 200 conferences and workshops. He has co-authored several book chapters and been awarded four patents. His Google Scholar h-index is 29, and his citations have exceeded 2700.
Author
Univ. of Kent (United Kingdom)
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NKT Photonics A/S (Denmark)
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Institut du Cerveau et de la Moelle Épinière (France)
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Univ. of Kent (United Kingdom)
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NKT Photonics A/S (Denmark)
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NKT Photonics A/S (Denmark)
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Technical Univ. of Denmark (Denmark)
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Technical Univ. of Denmark (Denmark)
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Univ. of Kent (United Kingdom)
Presenter/Author
Univ. of Kent (United Kingdom)