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11-16 April 2026
Conference 13006 > Paper 13006-43
Paper 13006-43

Comparison of soft tissue handling protocols for identifying optical properties and morphology

10 April 2024 • 13:40 - 14:00 CEST | Etoile B, Niveau/Level 1

Abstract

Sample handling is an important consideration when aiming for replicating in vivo conditions ex vivo for the sake of validating imaging protocols and identifying biomarkers of disease. We tested five different handling methods: Snap frozen in isopentane, directly frozen at -80 °C, slowly frozen in a cryobox with and without cryopreservation media, and formalin fixed. The samples were imaged using SD-OCT (1300nm) for qualitative and quantitative validation based on morphological and optical properties. All handling methods were compared in relation to representative histological image (HE staining), fresh tissue samples as well as each other. The results indicate a significant difference in the attenuation coefficient as well as morphological differences between the five different methods and support the hypothesis that proper sample handling is crucial for obtaining translatable results.

Presenter

Freja Høier
Technical Univ. of Denmark (Denmark)
Freja Høier is currently in the beginning of her second year on her PhD in biomedical optics in the Biophotonics Imaging group at the Technical University of Denmark in the Health Tech department. She has an interest in using multi-contrast imaging for detection of tumour demarcations and working for optimizing the imaging protocols and sample handling in order to translate her work into the clinic.
Application tracks: EU-funded Research
Presenter/Author
Freja Høier
Technical Univ. of Denmark (Denmark)
Author
Technical Univ. of Denmark (Denmark)
Author
Rigshospitalet (Denmark)
Author
Univ. of Copenhagen (Denmark)
Author
Andreas Kjær
Rigshospitalet (Denmark), Univ. of Copenhagen (Denmark)
Author
Technical Univ. of Denmark (Denmark)