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Conference 13006 > Paper 13006-103
Paper 13006-103

Broadband Coherent anti-Stokes Raman Spectroscopy for single cell hyperspectral imaging

9 April 2024 • 18:10 - 20:00 CEST | Galerie Schweitezer, Niveau/Level 0

Abstract

Hyperspectral imaging based on vibrational spectroscopy offers detailed, label-free chemical insights. Techniques like Near-Infrared (NIR), Fourier-Transform Infrared (FTIR), and Raman spectroscopy have bandwidth and speed constraints, particularly with water-based samples. Coherent Raman imaging, especially Stimulated Raman used with Second Harmonic Generation has recently been applied to produce virtual histology images without staining as well as segmenting cell features via spectral phasor analysis. Here we explore Broadband Coherent anti-Stokes Raman scattering (BCARS) as a method to produce high-quality virtually stained false-colour images of biological cells. BCARS enables fast, high-resolution (<10cm-1) and broad bandwidth (400-4000cm-1) bio-imaging but faces issues like low signal-to-noise ratio. This paper advances previous BCARS work, exploring new false-color image generation methods for diverse biological samples.

Presenter

Timothy J. McNamara
National Univ. of Ireland, Maynooth (Ireland)
Bryan Hennelly, with a BE and PhD from University College Dublin in electronic and optical engineering.,He established a biophotonics lab at Maynooth University focusing on quantittaive phase imaging and biomedical spectroscopy. His current research focuses on optoelectronic microscopy for clinical pathology, particularly in early-stage bladder cancer diagnosis using automated microscopy/spectroscopy systems inclusing spontaneous and coherent Raman spectrocopy. He has authored over 100 publications and collaborates extensively in academia, industry, and healthcare institutions.
Author
National Univ. of Ireland, Maynooth (Ireland)
Presenter/Author
Timothy J. McNamara
National Univ. of Ireland, Maynooth (Ireland)
Author
National Univ. of Ireland, Maynooth (Ireland)