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11-16 April 2026
Conference 13006 > Paper 13006-57
Paper 13006-57

Assessment of formation of Escherichia coli biofilm on solid substrates using laser speckle imaging parameters

11 April 2024 • 11:30 - 11:50 CEST | Etoile B, Niveau/Level 1

Abstract

Bacterial biofilms exist as aggregates of bacterial cells enclosed in a polysaccharide matrix, offering them resistance to various mechanical, chemical, antimicrobial, and environmental stress. The formation of biofilm on solid substrates occurs through a series of steps. It is essential to understand the stage of biofilm formation for several biomedical applications, such as assessing the coating efficacy for hard tissue implants, devising treatment strategies to inhibit biofilm formation in thin tissues, and building biocompatible diagnostic devices. In this work, we propose Laser Speckle Image Analysis to evaluate the bacterial biofilm formation stages with the help of different parameters extracted from the laser speckle image. These parameters give insight into the concentration and orientation changes of bacteria through the different stages of biofilm. The formation of biofilm on polypropylene substrate is evaluated and validated with optical microscopy images.

Presenter

Indian Institute of Technology Madras (India)
Ms.Priya Krishnamurthy is currently pursuing her PhD in the Department of Applied Mechanics and Biomedical Engineering at IIT Madras, India. She has completed her B.E. in Biomedical Engineering from PSG College of Technology, Coimbatore, India, and her M.S. in Biomedical Engineering from the Nanyang Technological University, Singapore. Her research interests include biomedical spectroscopy techniques and is currently working on laser speckle imaging to understand scattering in various biological media. Today she will be presenting her work on the formation of biofilm with the help of laser speckle imaging parameters.
Presenter/Author
Indian Institute of Technology Madras (India)
Author
Indian Institute of Technology Madras (India)
Author
Sathyabama Institute of Science and Technology (India)
Author
Sathyabama Institute of Science and Technology (India)