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11-16 April 2026
Conference 13006 > Paper 13006-61
Paper 13006-61

Algorithm for 10-fold faster flow cytometry light scattering measurements of extracellular vesicles

11 April 2024 • 14:20 - 14:40 CEST | Etoile B, Niveau/Level 1

Abstract

Flow cytometry (FCM) quantifies sub-µm particles in body fluids as biomarkers. Typical FCM electronics detect particles based on a light scattering signal exceeding a threshold. When the sample dilution is reduced to enhance statistics, simultaneous illumination, and false detections occur. To enable measurements at higher concentrations, a signal processing algorithm was developed. The algorithm was evaluated by measuring dilution series of 150 nm and a mixture (150, 200, and 300 nm) of polystyrene beads. FCM electronics reliably measured up to 6.7×E+7 〖ml〗^(-1) for 150 nm beads and 1.3×E+8 〖ml〗^(-1) for the mixture, while the algorithm achieved concentrations up to 1.5×E+9 〖(ml〗^(-1)), enabling 10-fold faster concentration measurements.

Presenter

Amsterdam UMC (Netherlands)
Mona Shahsavari is presently engaged in her Ph.D. studies within the Department of Biomedical Engineering and Physics at Amsterdam UMC - location AMC. Her research is focused on validating and applying innovative optical techniques for utilizing extracellular vesicles as biomarkers for diseases. She holds two MSc degrees, one in Biomechanical Engineering from École Polytechnique of Paris, and another in Fluid Mechanical Engineering from Sharif University in Iran.
Presenter/Author
Amsterdam UMC (Netherlands)
Author
Amsterdam UMC (Netherlands)
Author
Mendel Engelaer
Amsterdam UMC (Netherlands)
Author
Amsterdam UMC (Netherlands)
Author
Edwin van der Pol
Amsterdam UMC (Netherlands)