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Conference 13006 > Paper 13006-3
Paper 13006-3

Advancing hemoglobinopathy screening with Raman spectroscopy and machine learning

8 April 2024 • 12:10 - 12:30 CEST | Etoile B, Niveau/Level 1

Abstract

Hemoglobinopathies are among the most common inherited diseases worldwide, affecting approximately 7% of the global population. Despite advances in the standardization and harmonization of methods for HbA1c determination, an increasing number of hemoglobinopathies cause false HbA1c results. One of the common techniques for screening hemoglobinopathies is through high-performance liquid chromatography (HPLC) separation, followed by UV–VIS detection. Although UV–VIS can quantify the hemoglobin fractions, it is unable to identify them. In this study, we use Raman spectroscopy to study the fingerprint spectra of hemoglobin fractions based on which the fractions can be identified. To evaluate the potential of Raman spectroscopy in identifying these fractions, we utilize a range of commercially available hemoglobin fractions, including fetal hemoglobin. We automate the classification process with machine learning approaches such as support vector machines (SVM), fully connected neural networks (NN), k-Nearest Neighbors (KNN), Decision Trees (DT), and Bernoulli Naive Bayes (BNB). These models are fine-tuned and optimized to classify the hemoglobin fractions and achieve test accuracies of 98.2% and 98.5%, respectively. Our research highlights the potential of Raman spectroscopy as an identification tool when combined with HPLC.

Presenter

Vrije Univ. Brussel (Belgium)
Sara Abbasi is a doctorate candidate in Brussels Photonics (B-PHOT), part of the Applied Physics and Photonics Department of Vrije Universiteit Brussel (VUB). Her research focuses on the development of an optofluidic device designed for Raman spectroscopy, with the aim of improving the accuracy of diabetes monitoring.
Application tracks: AI/ML , EU-funded Research
Presenter/Author
Vrije Univ. Brussel (Belgium)
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Vrije Univ. Brussel (Belgium)
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Univ. Ziekenhuis Brussel (Belgium)
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Vrije Univ. Brussel (Belgium), Flanders Make (Belgium)
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Vrije Univ. Brussel (Belgium), Flanders Make (Belgium)
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Vrije Univ. Brussel (Belgium), Flanders Make (Belgium)
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Vrije Univ. Brussel (Belgium), Flanders Make (Belgium)