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Proceedings Paper

Colony image acquisition and genetic segmentation algorithm and colony analyses
Author(s): W. X. Wang
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Paper Abstract

Colony anaysis is used in a large number of engineerings such as food, dairy, beverages, hygiene, environmental monitoring, water, toxicology, sterility testing. In order to reduce laboring and increase analysis acuracy, many researchers and developers have made efforts for image analysis systems. The main problems in the systems are image acquisition, image segmentation and image analysis. In this paper, to acquire colony images with good quality, an illumination box was constructed. In the box, the distances between lights and dishe, camra lens and lights, and camera lens and dishe are adjusted optimally. In image segmentation, It is based on a genetic approach that allow one to consider the segmentation problem as a global optimization,. After image pre-processing and image segmentation, the colony analyses are perfomed. The colony image analysis consists of (1) basic colony parameter measurements; (2) colony size analysis; (3) colony shape analysis; and (4) colony surface measurements. All the above visual colony parameters can be selected and combined together, used to make a new engineeing parameters. The colony analysis can be applied into different applications.

Paper Details

Date Published: 24 January 2012
PDF: 13 pages
Proc. SPIE 8292, Color Imaging XVII: Displaying, Processing, Hardcopy, and Applications, 82921C (24 January 2012); doi: 10.1117/12.913588
Show Author Affiliations
W. X. Wang, Henan Polytechnic Univ. (China)

Published in SPIE Proceedings Vol. 8292:
Color Imaging XVII: Displaying, Processing, Hardcopy, and Applications
Reiner Eschbach; Gabriel G. Marcu; Alessandro Rizzi, Editor(s)

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