Share Email Print

Proceedings Paper

Dynamic multiphoton imaging of reversible and irreversible thermal changes in collagen tissues
Author(s): Vladimir A. Hovhannisyan; Ping-Jung Su; Chen-Yuan Dong
Format Member Price Non-Member Price
PDF $17.00 $21.00

Paper Abstract

Collagen is the major component of skin, tendon, cartilage, cornea, and, as a main structural protein it is the key determinant of thermo-mechanical properties of collagen-rich tissues in mammals. Thermal damage of chicken dermis and tendon, bovine leg tendon, and other collagen contained tissues were investigated with the use of second harmonic generation (SHG) and two-photon excited auto-fluorescence microscopy and spectroscopy. Samples were heating in a temperature-controlled water bath in the temperature range 18-90° C. SHG time-lapse imaging and analysis of intensity decay showed that the collagen thermal destruction depended on both temperature and heating time, and can be modeled by the Arrhenius equation. Temporal decay of SHG signal from the chicken dermis was single exponential during isothermal treatment at temperatures above 60º C that allowed to determine activation energy and frequency factor of skin collagen denaturation. Furthermore, two-exponential decay and partially reversible change in SHG intensity were registered during the tendon thermal treatment. A simple laser system and procedure is proposed for a real-time monitoring of collagen fiber thermal modification within a microscopic volume of 1 nl.

Paper Details

Date Published: 8 June 2011
PDF: 6 pages
Proc. SPIE 8087, Clinical and Biomedical Spectroscopy and Imaging II, 808710 (8 June 2011); doi: 10.1117/12.890023
Show Author Affiliations
Vladimir A. Hovhannisyan, National Taiwan Univ. (Taiwan)
Ping-Jung Su, National Taiwan Univ. (Taiwan)
Chen-Yuan Dong, National Taiwan Univ. (Taiwan)

Published in SPIE Proceedings Vol. 8087:
Clinical and Biomedical Spectroscopy and Imaging II
Nirmala Ramanujam; Jürgen Popp, Editor(s)

© SPIE. Terms of Use
Back to Top
Sign in to read the full article
Create a free SPIE account to get access to
premium articles and original research
Forgot your username?