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Proceedings Paper

Testing possibilities for establishing nanodiamond-DNA-conjugates
Author(s): A. Pohl; S. Joch; J. Michael; E. Boschke; P. Quenzel; J. Schreiber; V. Lapina; J. Opitz
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Paper Abstract

We report on the biofunctionalization of nanodiamond surfaces in a two step procedure: chemical modification, resulting in homogeneous and defined surface coating, with following addition of ssDNA. Carboxylation, thymidine coupling and amination methods for chemical modification of diamond surfaces for further functionalization experiments were applied. To enable the coupling process, biomolecules were also chemically modified with functional groups (-NH2). FTIR spectroscopy, fluorescence microscopy and gel electrophoresis were applied for characterizing modified ND particles and bioconjugates and for controlling the coupling success.

Paper Details

Date Published: 14 June 2011
PDF: 11 pages
Proc. SPIE 8087, Clinical and Biomedical Spectroscopy and Imaging II, 80871T (14 June 2011); doi: 10.1117/12.889859
Show Author Affiliations
A. Pohl, Fraunhofer-Institut für Zerstörungsfreie Prüfverfahren (Germany)
S. Joch, Technische Univ. Dresden (Germany)
J. Michael, Fraunhofer-Institut für Zerstörungsfreie Prüfverfahren (Germany)
E. Boschke, Technische Univ. Dresden (Germany)
P. Quenzel, Technische Univ. Dresden (Germany)
J. Schreiber, Fraunhofer-Institut für Zerstörungsfreie Prüfverfahren (Germany)
V. Lapina, B.I. Stepanov Institute of Physics (Belarus)
J. Opitz, Fraunhofer-Institut für Zerstörungsfreie Prüfverfahren (Germany)

Published in SPIE Proceedings Vol. 8087:
Clinical and Biomedical Spectroscopy and Imaging II
Nirmala Ramanujam; Jürgen Popp, Editor(s)

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