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Proceedings Paper

Fluorescence wavelength-time matrix acquisition for biomedical tissue diagnostics
Author(s): William R. Lloyd; Robert H. Wilson; Leng-Chun Chen; Gregory D. Gillispie; Mary-Ann Mycek
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Paper Abstract

A specialized transient digitizer system was developed for spectroscopic collection of fluorescence wavelength-time matrices (WTMs) from biological tissues. The system is compact, utilizes fiber optic probes for clinical compatibility, and offers rapid collection of high signal-to-noise ratio (>100) time- and wavelength- resolved fluorescence. The system is compatible with excitation sources operating in excess of 25 kHz. Wavelength-resolved measurement range is 300-800 nm with ≥ 0.01 nm steps. Time-resolved measurement depth is 128 ns with fixed 0.2 ns steps. The information-rich WTM data provides comprehensive fluorescence sensing capabilities, as demonstrated on tissue simulating phantoms. Extracting wavelength-resolved fluorophore lifetimes illustrates the potential of using the technology to resolve exogenous or endogenous fluorophore contributions in tissue samples in a clinical setting for tissue diagnostics and monitoring.

Paper Details

Date Published: 8 June 2011
PDF: 7 pages
Proc. SPIE 8087, Clinical and Biomedical Spectroscopy and Imaging II, 80870Y (8 June 2011); doi: 10.1117/12.889649
Show Author Affiliations
William R. Lloyd, Univ. of Michigan (United States)
Robert H. Wilson, Univ. of Michigan (United States)
Leng-Chun Chen, Univ. of Michigan (United States)
Gregory D. Gillispie, Fluorescence Innovations, Inc. (United States)
Mary-Ann Mycek, Univ. of Michigan (United States)

Published in SPIE Proceedings Vol. 8087:
Clinical and Biomedical Spectroscopy and Imaging II
Nirmala Ramanujam; Jürgen Popp, Editor(s)

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