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Proceedings Paper

The influence of 3d3 type transition metals on light scattering properties of sulfur cycle bacteria Desulfuromonas acetoxidans
Author(s): Oleksandr I. Bilyi; Oresta M. Vasyliv; Svitlana O. Hnatush; Vasyl B. Getman; Galyna I. Kotsyumbas
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Paper Abstract

Light scattering properties of bacterial cells mostly depend on their sizes, refractive indexes of their components and surrounding environment. Interaction between bacterial cells and 3d3 type transition metals causes their optical characteristics' changes. Desulfuromonas acetoxidans are uncolored gram-negative obligatory anaerobic sulfur reducing bacteria that can be used as microbial fuel cells with high electron recovery from different organic compounds oxidation to electric current as a result of electrons transfer in the processes of sulfur and some 3d3 type transition metals reduction, such as Ferrum and Manganese. In this study size distribution and relative content in the chosen interval of sizes (0.2 - 2.0 μm) of sulfur reducing D. acetoxidans bacterial cells under the influence of different concentrations of manganese chloride (II) hexahydrate, ferrous chloride (III) hexahydrate and ferrous sulfate (II) have been investigated by the new method of measuring. A method includes sounding of flow suspended bacterial cells by monochromatic coherent light, registration of signals of co-operation of sounding radiation with the explored microbiological objects by detects amplitudes and durations of scattered light impulses. Correlation between changes of light-scattering properties and growth of Desulfuromonas acetoxidans cells under these conditions has been shown.

Paper Details

Date Published: 14 June 2011
PDF: 6 pages
Proc. SPIE 8087, Clinical and Biomedical Spectroscopy and Imaging II, 808725 (14 June 2011); doi: 10.1117/12.889441
Show Author Affiliations
Oleksandr I. Bilyi, Ivan Franko National Univ. of L'viv (Ukraine)
Oresta M. Vasyliv, Ivan Franko National Univ. of L'viv (Ukraine)
Svitlana O. Hnatush, Ivan Franko National Univ. of L'viv (Ukraine)
Vasyl B. Getman, Ivan Franko National Univ. of L'viv (Ukraine)
Galyna I. Kotsyumbas, SSRCI of Veterinary Preparations and Fodder Additives (Ukraine)

Published in SPIE Proceedings Vol. 8087:
Clinical and Biomedical Spectroscopy and Imaging II
Nirmala Ramanujam; Jürgen Popp, Editor(s)

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