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Proceedings Paper

Cell death detection and ionic homeostasis monitoring with digital holographic microscopy
Author(s): Nicolas Pavillon; Jonas Kühn; Pascal Jourdain; Christian Depeursinge; Pierre J. Magistretti; Pierre Marquet
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Paper Abstract

Digital holographic microscopy is an interferometric technique enabling the measurement of the quantitative phase shifts induced by cell bodies. We correlate the phase signal measured on neurons with calcium imaging measured by fluorescence on cells loaded with Fluo-4, to monitor responses to glutamate challenges, which provoke well-known calcium increases through activation of various membrane receptors. A very good correspondence can be identified between the two signals, showing the links between the phase signal, being a measure of the intracellular dilution, and the calcium concentration within cells. We then check cell viability by employing propidium iodide (PI), a fluorescent indicator relying on the cell membrane integrity loss to assess cell death. Strong intracellular calcium concentration is indeed known to induce excitotoxic effects, potentially inducing cell death. This enables showing that some cells cannot sustain the calcium saturation identified in our measurements, leading to subsequent cell death.

Paper Details

Date Published: 14 June 2011
PDF: 6 pages
Proc. SPIE 8090, Novel Biophotonic Techniques and Applications, 809004 (14 June 2011); doi: 10.1117/12.889246
Show Author Affiliations
Nicolas Pavillon, Ecole Polytechnique Fédérale de Lausanne (Switzerland)
Jonas Kühn, Ecole Polytechnique Fédérale de Lausanne (Switzerland)
CHUV (Switzerland)
Pascal Jourdain, Ecole Polytechnique Fédérale de Lausanne (Switzerland)
Christian Depeursinge, Ecole Polytechnique Fédérale de Lausanne (Switzerland)
Pierre J. Magistretti, CHUV (Switzerland)
Ecole Polytechnique Fédérale de Lausanne (Switzerland)
Pierre Marquet, CHUV (Switzerland)
Ecole Polytechnique Fédérale de Lausanne (Switzerland)

Published in SPIE Proceedings Vol. 8090:
Novel Biophotonic Techniques and Applications
Henricus J. C. M. Sterenborg; I. Alex Vitkin, Editor(s)

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