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Proceedings Paper

Non-invasive SFG spectroscopy: a tool to reveal the conformational change of grafted chains due to bacterial adhesion
Author(s): Emilie Bulard; Henri Dubost; Marie-Pierre Fontaine-Aupart; Wanquan Zheng; Jean-Marie Herry; Marie-Noëlle Bellon-Fontaine; Romain Briandet; Bernard Bourguignon
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Paper Abstract

In many fields such as biomedical or food industry, surface colonization by micro-organisms leads to biofilms formation that are tridimentional biostructures highly resistant to the action of antimicrobials, by mechanisms still unclear. In order to deepen our understanding of the initial interaction of bacteria cells with a solid surface, we analyze by in situ vibrational Sum Frequency Generation (SFG) spectroscopy the effect of the adhesion of hydrophilic Lactoccocus lactis bacteria and its hydrophobic mutants in distilled water on a self-assembled monolayer (SAM) of octadecanethiol (ODT) on a gold film. When a homogeneous bacterial monolayer is deposited on this ordered surface, SFG spectrum of the ODT SAM shows significant intensity changes from that in air or in water. Its modelling as a function of conformation allows to distinguish optical effects due to the water solution surrounding bacteria from conformational changes of the ODT SAM due to the presence of the bacteria cells. Futhermore, bacterial adhesion induces different measurable effects on the ODT SAM conformation, depending on the hydrophobic / hydrophilic character of the bacterial surface. Such a result deserves to be taken into account for the design of new materials with improved properties or to control biofilm formation.

Paper Details

Date Published: 8 June 2011
PDF: 9 pages
Proc. SPIE 8087, Clinical and Biomedical Spectroscopy and Imaging II, 80870C (8 June 2011); doi: 10.1117/12.888786
Show Author Affiliations
Emilie Bulard, ISMO-CNRS, Univ. Paris-Sud 11 (France)
Henri Dubost, ISMO-CNRS, Univ. Paris-Sud 11 (France)
Marie-Pierre Fontaine-Aupart, ISMO-CNRS, Univ. Paris-Sud 11 (France)
Wanquan Zheng, ISMO-CNRS, Univ. Paris-Sud 11 (France)
Jean-Marie Herry, INRA-AgroParisTech (France)
Marie-Noëlle Bellon-Fontaine, INRA-AgroParisTech (France)
Romain Briandet, INRA-AgroParisTech (France)
Bernard Bourguignon, ISMO-CNRS, Univ. Paris-Sud 11 (France)

Published in SPIE Proceedings Vol. 8087:
Clinical and Biomedical Spectroscopy and Imaging II
Nirmala Ramanujam; Jürgen Popp, Editor(s)

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