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Proceedings Paper

Photodynamic therapy on bacterial reduction in dental caries: in vivo study
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Paper Abstract

The reduction of pathogenic microorganisms in supragingival plaque is one of the principal factors in caries prevention and control. A large number of microorganisms have been reported to be inactivated in vitro by photodynamic therapy (PDT). The purpose of this study was to develop a rat model to investigate the effects of PDT on bacterial reduction in induced dental caries. Twenty four rats were orally inoculated with Streptococcus mutans cells (ATCC 25175) for three consecutive days. The animals were fed with a cariogenic diet and water with 10% of sucrose ad libitum, during all experimental period. Caries lesion formation was confirmed by Optical Coherence Tomography (OCT) 5 days after the beginning of the experiment. Then, the animals were randomly divided into two groups: Control Group: twelve animals were untreated by either light or photosensitizer; and PDT Group: twelve animals were treated with 100μM of methylene blue for 5min and irradiated by a Light Emitting Diode (LED) at λ = 640±30nm, fluence of 172J/cm2, output power of 240mW, and exposure time of 3min. Microbiological samples were collected before, immediately after, 3, 7 and 10 days after treatment and the number of total microaerophiles was counted. OCT images showed areas of enamel demineralization on rat molars. Microbiological analysis showed a significant bacterial reduction after PDT. Furthermore, the number of total microaerophiles in PDT group remained lower than control group until 10 days posttreatment. These findings suggest that PDT could be an alternative approach to reduce bacteria in dental caries.

Paper Details

Date Published: 17 May 2010
PDF: 6 pages
Proc. SPIE 7715, Biophotonics: Photonic Solutions for Better Health Care II, 77151R (17 May 2010); doi: 10.1117/12.854597
Show Author Affiliations
Alessandra Baptista, Instituto de Pesquisas Energéticas e Nucleares (Brazil)
Renato Araujo Prates, Instituto de Pesquisas Energéticas e Nucleares (Brazil)
Ilka Tiemy Kato, Instituto de Pesquisas Energéticas e Nucleares (Brazil)
Marcello Magri Amaral, Instituto de Pesquisas Energéticas e Nucleares (Brazil)
Anderson Zanardi de Freitas, Instituto de Pesquisas Energéticas e Nucleares (Brazil)
Martha Simões Ribeiro, Instituto de Pesquisas Energéticas e Nucleares (Brazil)


Published in SPIE Proceedings Vol. 7715:
Biophotonics: Photonic Solutions for Better Health Care II
Jürgen Popp; Wolfgang Drexler; Valery V. Tuchin; Dennis L. Matthews, Editor(s)

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