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Proceedings Paper

Raman microscopy of individual living human embryonic stem cells
Author(s): S. M. Novikov; J. Beermann; S. I. Bozhevolnyi; L. M. Harkness; M. Kassem
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Paper Abstract

We demonstrate the possibility of mapping the distribution of different biomolecules in living human embryonic stem cells grown on glass substrates, without the need for fluorescent markers. In our work we improve the quality of measurements by finding a buffer that gives low fluorescence, growing cells on glass substrates (whose Raman signals are relatively weak compared to that of the cells) and having the backside covered with gold to improve the image contrast under direct white light illumination. The experimental setup used for Raman microscopy is the commercially available confocal scanning Raman microscope (Alpha300R) from Witec and sub-μm spatially resolved Raman images were obtained using a 532 nm excitation wavelength.

Paper Details

Date Published: 18 May 2010
PDF: 5 pages
Proc. SPIE 7715, Biophotonics: Photonic Solutions for Better Health Care II, 771537 (18 May 2010);
Show Author Affiliations
S. M. Novikov, Univ. of Southern Denmark (Denmark)
J. Beermann, Univ. of Southern Denmark (Denmark)
S. I. Bozhevolnyi, Univ. of Southern Denmark (Denmark)
L. M. Harkness, Univ. Hospital of Odense (Denmark)
M. Kassem, Univ. Hospital of Odense (Denmark)

Published in SPIE Proceedings Vol. 7715:
Biophotonics: Photonic Solutions for Better Health Care II
Jürgen Popp; Wolfgang Drexler; Valery V. Tuchin; Dennis L. Matthews, Editor(s)

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