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Proceedings Paper

Two channel microfluidic CARS for quantifying pure vibrational contrast of model analytes
Author(s): B. Dietzek; G. Bergner; D. Akimov; D. Malsch; T. Henkel; S. Schlücker; J. Popp
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Paper Abstract

We discuss the combination of a CARS-imaging system with microfluidics. Such system is a versatile tool to quantify the relative contributions of resonant and non-resonant scattering at the CARS frequency. We will show that the twochannel microfluidic chip employed in combination with deuterated isotopomers as an internal standard allows for fast and quantitative detection of organic molecules by CARS microscopy. The experimental design enables the simultaneous measurement of both the chemically relevant Raman-resonant signal and the non-Raman-resonant background.

Paper Details

Date Published: 28 April 2010
PDF: 9 pages
Proc. SPIE 7715, Biophotonics: Photonic Solutions for Better Health Care II, 77152X (28 April 2010); doi: 10.1117/12.853843
Show Author Affiliations
B. Dietzek, IPHT Jena (Germany)
Friedrich-Schiller-Univ. Jena (Germany)
G. Bergner, IPHT Jena (Germany)
Univ. Osnabrück (Germany)
D. Akimov, IPHT Jena (Germany)
D. Malsch, IPHT Jena (Germany)
T. Henkel, IPHT Jena (Germany)
S. Schlücker, Univ. Osnabrück (Germany)
J. Popp, IPHT Jena (Germany)
Friedrich-Schiller Univ. Jena (Germany)

Published in SPIE Proceedings Vol. 7715:
Biophotonics: Photonic Solutions for Better Health Care II
Jürgen Popp; Wolfgang Drexler; Valery V. Tuchin; Dennis L. Matthews, Editor(s)

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