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Proceedings Paper

Multiplex detection of disease marker proteins with arrayed imaging reflectometry
Author(s): Amrita Yadav; Rashmi Sriram; Benjamin L. Miller
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Paper Abstract

Arrayed Imaging Reflectometry, or "AIR", is a new label-free optical technique for detecting proteins. AIR relies on binding-induced changes in the response of an antireflective coating on the surface of a silicon chip. Thus far, we have demonstrated the use of AIR for the detection of pathogenic E. coli, and for multiplex detection of a broad range of proteins in human serum. Creation of the near-perfect antireflective coating on the surface of silicon requires careful control over preparation of the chip surface prior to probe molecule immobilization. We present methods for highly reproducible, solution-phase silanization and glutaraldehyde functionalization of silicon chips carrying a layer of thermal oxide. Following functionalization with antibodies and passivation of remaining reactive groups, these surfaces provide exceptional performance in the AIR assay.

Paper Details

Date Published: 17 February 2010
PDF: 6 pages
Proc. SPIE 7553, Frontiers in Pathogen Detection: From Nanosensors to Systems, 75530J (17 February 2010);
Show Author Affiliations
Amrita Yadav, Univ. of Rochester (United States)
Rashmi Sriram, Univ. of Rochester (United States)
Benjamin L. Miller, Univ. of Rochester (United States)

Published in SPIE Proceedings Vol. 7553:
Frontiers in Pathogen Detection: From Nanosensors to Systems
Philippe M. Fauchet; Benjamin L. Miller, Editor(s)

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