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Proceedings Paper

Spatiofrequency filters for imaging fluorescence in scattering media
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Paper Abstract

Researchers have been using simple optics to image optically induced fluorescence in tissues. We now apply the Angular Domain Imaging technique using a Spatiofrequency filter which accepts only photons within a small deviation angle from its original trajectory to image a fluorescing medium beneath a scattering layer. A Rhodamine 6 G dye fluorescing layer or fluorescence slides, under an Intralipid scattering medium was used. By applying ADI with acceptance angle of 0.17°, the structures are distinguishable at low scattering depth depending of the emission wavelength of the fluorescence source. It was established previously that as the acceptance angle increases, the amount of scattered light/noise in the images increases, however, the resolution also deteriorates. Simulations using a Monte-Carlo program are done for both angular filters, Spatiofrequency filter and Linear Collimating Array. Due to the additional positional filtration on top of the angular filtration with Linear Collimating Array, collimators with aspect ratio as low as 10:1 can improve the quality of the fluorescence images significantly in both contrast ratio and resolution.

Paper Details

Date Published: 22 February 2010
PDF: 12 pages
Proc. SPIE 7562, Optical Interactions with Tissues and Cells XXI, 75620B (22 February 2010); doi: 10.1117/12.842998
Show Author Affiliations
Polly Tsui, Simon Fraser Univ. (Canada)
Glenn H. Chapman, Simon Fraser Univ. (Canada)
Rongen L. K. Cheng, Simon Fraser Univ. (Canada)
Gary Chiang, Simon Fraser Univ. (Canada)
Nick Pfeiffer, Simon Fraser Univ. (Canada)
Bozena Kaminska, Simon Fraser Univ. (Canada)

Published in SPIE Proceedings Vol. 7562:
Optical Interactions with Tissues and Cells XXI
E. Duco Jansen; Robert J. Thomas, Editor(s)

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