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Proceedings Paper

The effect of temperature of fluorescence: an animal study
Author(s): Alex Walsh; Bart Masters; Duco Jansen; A. J. Welch; Anita Mahadevan-Jansen
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Paper Abstract

The effect of temperature on the fluorescence of enucleated porcine eyes and rat skin was studied. The fluorescence peak intensity was found to decrease as the tissue temperature increased. A dual-excitation, fiber-based system was used to collect fluorescence and diffuse-reflectance spectra from the samples. A thermal camera was used to determine the temperature of the tissue at the time of fluorescence measurement. The samples were mounted in a saline bath and measurements were made as the tissue temperature was increased from -20°C to 70°C. Results indicate that temperature affects several fluorescence spectra characteristics. The peak height decreased as temperature increased. At temperatures above 60°C, the peak position shifted to lower wavelengths. Heating and cooling experiments of the rat skin demonstrate the recovery of the loss in fluorescence. The diffuse reflectance spectra indicated a change in optical properties past 60°C, but prior to the denaturation temperature for collagen at 57°C, no change in optical properties was observed. Results suggest that the decrease in fluorescence is both a property of fluorescence and a result of altering optical properties.

Paper Details

Date Published: 11 February 2010
PDF: 5 pages
Proc. SPIE 7562, Optical Interactions with Tissues and Cells XXI, 75620W (11 February 2010); doi: 10.1117/12.842578
Show Author Affiliations
Alex Walsh, Vanderbilt Univ. (United States)
Bart Masters, Vanderbilt Univ. (United States)
Duco Jansen, Vanderbilt Univ. (United States)
A. J. Welch, The Univ. of Texas at Austin (United States)
Anita Mahadevan-Jansen, Vanderbilt Univ. (United States)

Published in SPIE Proceedings Vol. 7562:
Optical Interactions with Tissues and Cells XXI
E. Duco Jansen; Robert J. Thomas, Editor(s)

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