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Proceedings Paper

Lung alveolar wall disruption in three-dimensional space identified using second-harmonic generation and multiphoton excitation fluorescence
Author(s): Thomas Abraham; James Hogg M.D.
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Paper Abstract

Second harmonic generation and multiphoton excited fluorescence microscopy methods were used to examine structural remodeling of the extracellular matrix in human lung alveolar walls undergoing emphysematous destruction. Fresh lung samples removed from a patient undergoing lung transplantation for very severe chronic obstructive pulmonary disease were compared to similar samples from an unused donor lung that served as a control. The generated spatially resolved 3D images show the spatial distribution of collagen, elastin and other endogenously fluorescent tissue components such as macrophages. In the case of control lung tissue, we found well ordered alveolar walls with composite type structure made up of collagen matrix and relatively fine elastic fibers. In contrast, lung tissue undergoing emphysematous destruction was highly disorganized with increased alveolar wall thickness compared to control lung tissue.

Paper Details

Date Published: 22 February 2010
PDF: 9 pages
Proc. SPIE 7555, Advanced Biomedical and Clinical Diagnostic Systems VIII, 75550X (22 February 2010); doi: 10.1117/12.842321
Show Author Affiliations
Thomas Abraham, Univ. of British Columbia (Canada)
James Hogg M.D., Univ. of British Columbia (Canada)

Published in SPIE Proceedings Vol. 7555:
Advanced Biomedical and Clinical Diagnostic Systems VIII
Tuan Vo-Dinh; Warren S. Grundfest M.D.; Anita Mahadevan-Jansen, Editor(s)

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