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Proceedings Paper

Investigation of a tissue engineered tendon model by PS-OCT
Author(s): Ying Yang; Mark Ahearne; Ian Wimpenny; Juan Guijarro-Leach; Jim Torbet
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Paper Abstract

A few native tissues, such as tendon, skin and eye, possess highly organized collagenous matrices. In particular, the collagen fibers in tendon are organized into a hierarchical and unidirectional format, which gives rise to the high tissuespecific mechanical properties. This organization has been clearly revealed by a conventional polarized light microscope. The newly developed polarization-sensitive optical coherence tomography (PS-OCT) technique allows non-invasive visualization of birefringence images arising from orientated structures in a three dimensional format. Our previous studies of native tendon and tissue engineered tendon by PS-OCT demonstrate that tissue engineered tendon has a far less perfect collagen fiber organization than native tendon even under dynamic culture conditions. The purpose of this study is to use PS-OCT to assess the relationship between the degree of birefringence, collagen concentration and fiber density in model tendon tissues. The model tissue is constructed from an aligned collagen hydrogel and aligned polyester nanofibers. The effects of the diameter and density of the nanofibers and the collagen concentration in the model have been investigated. The alignment of collagen fibrils is induced by application of a high magnetic field during fibrillogenesis while aligned polyester nanofibers are manufactured using the electrospinning technique. It is found that the collagen concentration, the density and size of nanofiber bundles are the key parameters to produce birefringence in OCT images. The perfectly aligned collagen hydrogel with concentration as high as 4 mg/ml does not exhibit a birefringence image until the hydrogel has been compressed and concentrated. Aligned nanofiber bundles have demonstrated marginal birefringence in the absence of the collagen matrix. These studies enhance our understanding of how to control and optimize the parameters in tendon tissue engineering.

Paper Details

Date Published: 24 February 2010
PDF: 5 pages
Proc. SPIE 7566, Optics in Tissue Engineering and Regenerative Medicine IV, 75660A (24 February 2010); doi: 10.1117/12.842302
Show Author Affiliations
Ying Yang, Keele Univ. (United Kingdom)
Mark Ahearne, Keele Univ. (United Kingdom)
Ian Wimpenny, Keele Univ. (United Kingdom)
Juan Guijarro-Leach, Keele Univ. (United Kingdom)
Jim Torbet, CREATA, CNRS (France)

Published in SPIE Proceedings Vol. 7566:
Optics in Tissue Engineering and Regenerative Medicine IV
Sean J. Kirkpatrick; Ruikang Wang, Editor(s)

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