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Proceedings Paper

Minimal invasive localization of the germinal disc in ovo for subsequent chicken sexing using optical coherence tomography
Author(s): Anke Burkhardt; Stefan Geissler; Peter Cimalla; Julia Walther; Edmund Koch
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Paper Abstract

Reason for using optical coherence tomography (OCT) to locate the germinal disc is the questionable and ethically alarming killing of male layer chickens because for the layer line only the females are necessary. To avoid this and to protect the animal rights, the sex of the fertilized chicken egg has to be determined as early as possible in the unincubated state. Because the information whether the chick becomes male or female can be found in the germinal disc an accurate localization for sexing is essential. The germinal disc is located somewhere on top of the yolk and has a diameter of approximately 4 - 5 mm. Different imaging methods like ultrasonography, 3D-X-ray micro computed tomography and magnetic resonance imaging were used for localization until now, but found to be impractical. The goal of this study is to prove if OCT can be a moderate approach for the precise in ovo localization. Because the eggshell is an impenetrable barrier for OCT and to minimize the penetration of germs a very small hole is placed in the eggshell and a fan-shaped optical scanning pattern is used.

Paper Details

Date Published: 19 February 2010
PDF: 5 pages
Proc. SPIE 7554, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XIV, 75542N (19 February 2010); doi: 10.1117/12.839463
Show Author Affiliations
Anke Burkhardt, Univ. of Technology Dresden (Germany)
Stefan Geissler, Univ. of Technology Dresden (Germany)
Peter Cimalla, Univ. of Technology Dresden (Germany)
Julia Walther, Univ. of Technology Dresden (Germany)
Edmund Koch, Univ. of Technology Dresden (Germany)

Published in SPIE Proceedings Vol. 7554:
Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XIV
Joseph A. Izatt; James G. Fujimoto; Valery V. Tuchin, Editor(s)

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