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Proceedings Paper

Salivary diagnostics: a new solution for an old problem: breast cancer detection
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Paper Abstract

Objective: The objective of this study is to determine if LC/MS/MS based isotopic tagging technique is useful to detect putative breast cancer markers in saliva. Methods: Six pooled (n=10 subjects per pool) stimulated whole saliva specimens from women were analyzed. One pooled specimen was from healthy women, another pooled specimen from women diagnosed with a benign breast tumor and the other four-pooled specimens were from women diagnosed with carcinoma of the breast. The four cancer specimens were staged 0, I, IIa and IIb (lymph node involvement). Isotopically tagging proteins in the tumor groups and comparing them to the healthy control group measured differential expression of proteins. The iTRAQ labels are isobaric and chemically identical, thus labeled peptides from the different pools have identical elution times and masses. The relative concentration of identified proteins in each of the mixtures is determined from the intensities of each of the reporter ions. Results: The results of the salivary analyses yielded approximately 209 proteins in the saliva specimens. These proteins were able to distinguish between the healthy control, the benign and the cancer patients. Additionally, there were proteins able to distinguish between node positive and node negative patients.

Paper Details

Date Published: 5 May 2009
PDF: 7 pages
Proc. SPIE 7306, Optics and Photonics in Global Homeland Security V and Biometric Technology for Human Identification VI, 730608 (5 May 2009); doi: 10.1117/12.820117
Show Author Affiliations
Charles F. Streckfus, Univ. of Texas Dental Branch at Houston (United States)

Published in SPIE Proceedings Vol. 7306:
Optics and Photonics in Global Homeland Security V and Biometric Technology for Human Identification VI
B.V.K. Vijaya Kumar; Craig S. Halvorson; Šárka O. Southern; Salil Prabhakar; Arun A. Ross, Editor(s)

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