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Proceedings Paper

Saliva-based system for health and toxicology monitoring
Author(s): D. B. Fenner; A. E. Stevens; D. I. Rosen; A. A. Ferrante; S. J. Davis
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Paper Abstract

The practical utility of technologies for early detection of human exposure to a variety of toxic agents has been limited in many cases by the absence of instruments suitable for first responders and at field hospitals. Microarrays provide multiplexed assay of a large number of human biomarkers, including cytokines and chemokines, indicators of immune system health. Assay of saliva is less invasive and provides quick indication of exposure especially of the respiratory system. Our pilot clinical study has uncovered an early cytokine response in human saliva. As a model for respiratory exposure, a cohort of 16 adult volunteers was challenged with FluMistTM vaccinations, an FDA approved, attenuated live influenza virus. Blood and saliva cytokine levels were monitored immediately prior to and up to 7 days afterwards. Bead assay found little change in blood cytokine levels while several of those in saliva were frequently elevated above two standard deviations on trial days one and three. We have developed a prototype portable saliva monitoring system consisting of microarray cytokine capture plate, luminescent reporter, and whole plate imaging. Assay is with a commercial 96-well plate spotted with up to 16 distinct biomarkers per well and read by chemiluminescence. A battery-powered, 16-bit, cooled-CCD camera and laptop PC provide imaging and data reduction. Detection limits of common inflammatory cytokines were measured at about 1-5 pg/ml which is within the clinically significant range for saliva of exposed individuals, as verified for samples from the small clinical trial. An expanded study of cytokine response in saliva of therapeutic radiation oncology patients is being launched.

Paper Details

Date Published: 5 May 2009
PDF: 7 pages
Proc. SPIE 7306, Optics and Photonics in Global Homeland Security V and Biometric Technology for Human Identification VI, 73060B (5 May 2009); doi: 10.1117/12.818834
Show Author Affiliations
D. B. Fenner, Physical Sciences, Inc. (United States)
A. E. Stevens, Physical Sciences, Inc. (United States)
D. I. Rosen, Physical Sciences, Inc. (United States)
A. A. Ferrante, Physical Sciences, Inc. (United States)
S. J. Davis, Physical Sciences, Inc. (United States)


Published in SPIE Proceedings Vol. 7306:
Optics and Photonics in Global Homeland Security V and Biometric Technology for Human Identification VI
B.V.K. Vijaya Kumar; Craig S. Halvorson; Šárka O. Southern; Salil Prabhakar; Arun A. Ross, Editor(s)

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