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Proceedings Paper

A fluorescence diagnostic system detecting cancer-specific enzymatic activities: preliminary results
Author(s): Tobias Beck; Herbert Stepp; Rainer Wittig; Markus Boehl; Peter Schubert; Sigrun Henkenjohann; Markus Sauer; Axel Duerkop; Christian S. Betz; Ann Johansson
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Paper Abstract

Colorectal carcinoma is one of the most frequent and deadliest tumors in the western world. The visualization of cancer-specific enzymatic activities could possibly improve sensitivity and specificity as compared to classical white-light endoscopy. DNase X, which is typically found in early lesions, and TKTL1, which identifies aggressive carcinomas with a high metastatic potential, could potentially constitute such cancer-specific enzymes. Here, fluorescent dyes have been developed in order to specifically detect these enzymatic activities. A fiber-based system was developed for the detection of small concentrations of fluorescent dyes in scattering and absorbing media. With the use of the reflectance spectrum and a theoretical model for the light distribution, the intrinsic fluorescence is assessed from the raw fluorescence. The resulting intrinsic spectrum shows only a weak dependence on the optical properties of the sample and its intensity correlates with the fluorophore concentration. Thus, small concentrations and small variations in the concentrations of the fluorescent dye can be measured. In conclusion, the presented fluorescence diagnostic system in combination with new fluorescent probes has the potential to distinguish between cancerous tissue samples with high enzymatic activity and non-cancerous tissue samples with lower enzymatic activity.

Paper Details

Date Published: 23 February 2009
PDF: 6 pages
Proc. SPIE 7169, Advanced Biomedical and Clinical Diagnostic Systems VII, 71691G (23 February 2009); doi: 10.1117/12.812958
Show Author Affiliations
Tobias Beck, Univ. Clinic Munich (Germany)
Herbert Stepp, Univ. Clinic Munich (Germany)
Rainer Wittig, Institut fuer Lasertechnologien in der Medizin und Messtechnik an der Univ. Ulm (Germany)
Markus Boehl, R-Biopharm AG (Germany)
Peter Schubert, R-Biopharm AG (Germany)
Sigrun Henkenjohann, Bielefeld Univ. (Germany)
Markus Sauer, Bielefeld Univ. (Germany)
Axel Duerkop, Univ. of Regensburg (Germany)
Christian S. Betz, Univ. Clinic Munich (Germany)
Ann Johansson, Univ. Clinic Munich (Germany)

Published in SPIE Proceedings Vol. 7169:
Advanced Biomedical and Clinical Diagnostic Systems VII
Anita Mahadevan-Jansen; Tuan Vo-Dinh; Warren S. Grundfest M.D., Editor(s)

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