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Proceedings Paper

Development of a targeted CT contrast agent: assessment of cellular interactions using novel integrated optical labels
Author(s): Naomi Matsuura; Melissa L. Hill; Ivan Gorelikov; Siqi Zhu; Kelvin Wan; James G. Mainprize; Martin J. Yaffe; J. A. Rowlands
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Paper Abstract

Computed tomography (CT) enables high resolution, whole-body imaging with excellent depth penetration. The development of new targeted radiopaque CT contrast agents can provide the required sensitivity and localization for the successful detection and diagnosis of smaller lesions representing earlier disease. Nanoscale, perfluorooctylbromide (C8F17Br, PFOB) droplets have previously been used as untargeted contrast agents in X-ray imaging, and form the basis of a promising new group of agents that can be developed for targeted CT imaging. For successful targeting to disease sites, new PFOB droplet formulations tailored for ideal in vivo performance (e.g., biodistribution, toxicity, and pharmacokinetics) must be developed. However, the direct assessment of PFOB agents in biological environments early in their development is difficult using CT, as its sensitivity is not adequate for identification of single probes in vitro or in vivo. In order to allow single droplet interactions with cells to be directly assessed using standard cellular imaging tools, we integrate an optical marker within the PFOB agent. In this work, a new method to label a PFOB agent with fluorescent quantum dot (QD) nanoparticles is presented. These composite PFOB-QD droplets loaded into macrophage cells result in fluorescence on a cellular level that correlates well to the strong CT contrast exhibited in corresponding tissue-mimicking cell pellets. QD loading within the PFOB droplet core allows optical labeling without influencing the surface-dependent properties of the PFOB droplets in vivo, and may be used to follow PFOB localization from in vitro cell studies to histopathology.

Paper Details

Date Published: 27 February 2009
PDF: 8 pages
Proc. SPIE 7262, Medical Imaging 2009: Biomedical Applications in Molecular, Structural, and Functional Imaging, 72622A (27 February 2009); doi: 10.1117/12.812048
Show Author Affiliations
Naomi Matsuura, Sunnybrook Health Sciences Ctr. (Canada)
Melissa L. Hill, Sunnybrook Health Sciences Ctr. (Canada)
Ivan Gorelikov, Sunnybrook Health Sciences Ctr. (Canada)
Siqi Zhu, Sunnybrook Health Sciences Ctr. (Canada)
Kelvin Wan, Sunnybrook Health Sciences Ctr. (Canada)
James G. Mainprize, Sunnybrook Health Sciences Ctr. (Canada)
Martin J. Yaffe, Sunnybrook Health Sciences Ctr. (Canada)
J. A. Rowlands, Sunnybrook Health Sciences Ctr. (Canada)

Published in SPIE Proceedings Vol. 7262:
Medical Imaging 2009: Biomedical Applications in Molecular, Structural, and Functional Imaging
Xiaoping P. Hu; Anne V. Clough, Editor(s)

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