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Proceedings Paper

A sandwich assay for procalcitonin detection for POCT applications
Author(s): Francesco Baldini; Luca Bolzoni; Ambra Giannetti; Melanie Kess; Petra M. Kraemer; Elisabeth Kremmer; Giampiero Porro; Folco Senesi; Cosimo Trono
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Paper Abstract

A plastic biochip was developed for the detection of procalcitonin (PCT) and consists of a polymethylmethacrylate (PMMA) chip shaped in order to achieve several flow microchannels. A sandwich assay using a new antibody pairs is implemented with the capture antibody immobilized on the PMMA surface and the target antibody labelled with a fluorophore. A laser diode excites the fluorescent sensing layer. Thanks to the anisotropy of the fluorescence the emitted light travels along the thickness of the plastic material. The fluorescence coming out from the chip is collected by 1 mm plastic optical fibre and detected with a spectrum analyser.

Paper Details

Date Published: 23 February 2009
PDF: 8 pages
Proc. SPIE 7169, Advanced Biomedical and Clinical Diagnostic Systems VII, 716905 (23 February 2009); doi: 10.1117/12.810265
Show Author Affiliations
Francesco Baldini, Institute of Applied Physics, CNR (Italy)
Luca Bolzoni, Datamed S.r.L. (Italy)
Ambra Giannetti, Institute of Applied Physics, CNR (Italy)
Melanie Kess, Helmholtz Zentrum München, Institute of Ecological Chemistry (Germany)
Petra M. Kraemer, Helmholtz Zentrum München, Institute of Ecological Chemistry (Germany)
Elisabeth Kremmer, Helmholtz Zentrum München, Institute for Molecular Immunology (Germany)
Giampiero Porro, Datamed S.r.L. (Italy)
Folco Senesi, Institute of Applied Physics, CNR (Italy)
Cosimo Trono, Institute of Applied Physics, CNR (Italy)


Published in SPIE Proceedings Vol. 7169:
Advanced Biomedical and Clinical Diagnostic Systems VII
Anita Mahadevan-Jansen; Tuan Vo-Dinh; Warren S. Grundfest M.D., Editor(s)

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